Effect of glutamine on liver injuries induced by intestinal ischemia-reperfusion in rats

Renata Hartmann, Francielli Licks, Elizângela Gonçalves Schemitt, Josieli Raskopf Colares, Juliana da Silva, Rosa Maria Moura, Gilmara Pandolfo Zabot, Henrique Sarubbi Fillmann, Norma Possa Marroni


Introduction: Intestinal ischemia-reperfusion (I/R) injury may cause cell and tissue damage, reaching also other organs such as the liver. Because of the involvement of free radicals in I/R injury, treatment options with antioxidants have been studied and tested.

Objective: To evaluate the effect of glutamine (Gln) in the liver of animals with intestinal I/R injury.

Methods: We used 20 male Wistar rats divided into four groups: sham-operated (SO); glutamine + sham-operated (G+SO); intestinal ischemia-reperfusion (I/R); glutamine + intestinal ischemia-reperfusion (G+I/R). The superior mesenteric artery was clamped for 30 minutes and reperfused for 15 minutes. Gln (25 mg/kg/day) diluted in 1 ml of saline was administered intraperitoneally on the two days before I/R induction.

Results: Levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), lipid peroxidation
(LPO) and expressions of interleukin-6 (IL-6) and nuclear factor kappa B (NF-kB) showed a significant reduction in the G+I/R group as compared with the I/R group. The activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and the levels of glutathione (GSH) showed an increase in the G+I/R group as compared with the I/R group.

Conclusion: Pretreatment with Gln reduced oxidative, tissue damage and showed a decrease expression of inflammatory mediators.

Palabras clave

DNA damage. Inflammation. Lipid peroxidation. Oxidative stress.

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DOI: http://dx.doi.org/10.20960/nh.643

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